Fig. 5. Effects of EHE on abdominal fat tissue morphology and expression of mRNA in the adipocyte differentiation-related genes in abdominal adipose tissues of HFD-fed mice.
(A) Abdominal fat tissue histology. Representative adipose tissue sections stained with H&E (200× magnification) show adipocyte sizes. (B) Average diameter of adipocytes in adipose tissues from each group. Adipocyte RNA was isolated, and real-time PCR analysis was performed to determine mRNA expressions of (C) ACS1, (D) FAS, (E) FATP1, and (F) perilipin. GAPDH was used as an internal control. All data are expressed as the mean ± SD of the experiment. #p < 0.05 and ##p < 0.01 compared to the LFD group; *p < 0.05 and **p < 0.01 compared to the HFD group.