Fig. 3. Effects of EHE and quercetin on the viability and differentiation of 3T3-L1 preadipocytes.
(A) MTT assay results show cell viability after treatment with EHE (50, 100, and 200 μg/ml) and quercetin (50 μg/ml) for 24 h. (B) Images show Oil Red O stained cells (200 × magnification). Graph shows the quantification of intracellular lipids. Adipogenesis was induced with IBMX, dexamethasone, and insulin (MDI). EHE and quercetin treatments inhibited lipid accumulation. The data are presented as the mean percentage compared to control DMSO-treated cells (MDI). All data are expressed as the mean ± SD of the experiment. ***p < 0.001 compared to the MDI control group.