HPLC conditions for analyzing the quercetin content in EHE.
Parameter | Conditions | ||
---|---|---|---|
Column | Agilent ZORBAX SB-C18 (4.6 mm × 250 mm, 5.0 μm, Agilent) |
||
Column temp. | Room temperature | ||
Time (min) | A |
B |
|
0 | 95 | 5 | |
5 | 75 | 25 | |
Mobile phase (Gradient) |
15 | 75 | 25 |
25 | 50 | 50 | |
28 | 40 | 60 | |
33 | 30 | 70 | |
40 | 95 | 5 | |
Detector | Shimadzu, SPD-M20A Diode Array Detector (334 nm) | ||
Flow rate | 1.0 ml/min | ||
Injection volume | 10 μl | ||
Run time | 40 min |
10.1% Phosphoric acid
2Acetonitrile