JMB

eISSN 1738-8872
pISSN 1017-7825

Table. 1.

Table. 1.

HPLC conditions for analyzing the quercetin content in EHE.

Parameter Conditions
Column Agilent ZORBAX SB-C18
(4.6 mm × 250 mm, 5.0 μm, Agilent)
Column temp. Room temperature
Time (min) A1 (%) B2 (%)
0 95 5
5 75 25
Mobile phase
(Gradient)
15 75 25
25 50 50
28 40 60
33 30 70
40 95 5
Detector Shimadzu, SPD-M20A Diode Array Detector (334 nm)
Flow rate 1.0 ml/min
Injection volume 10 μl
Run time 40 min

10.1% Phosphoric acid

2Acetonitrile

J. Microbiol. Biotechnol. 2024;34:387~398 https://doi.org/10.4014/jmb.2308.08004
© J. Microbiol. Biotechnol.