Fig. 8.

Fig. 8. Effects of HLT-101 on proliferation and apoptosis in BPH. (A) Western blot showing expression of proliferation-related proteins in prostate tissues of rat. Quantitative histogram of protein bands for the expression of (B) PCNA, (C) Cyclin D1 in prostate tissues of rat. (D) Western blot showing expression of apoptosis-related proteins in TP-induced BPH rats. Quantitative histogram of protein bands for the expression of (E) BAX/BCL-2 in prostate tissues of rat. Con, corn oil, subcutaneous injection (s.c.) and distilled water peroral (p.o.); BPH, TP (3 mg/kg, s.c.) and distilled water (p.o.); BPH + HLT- 101, TP (3 mg/kg, s.c.), and HLT-101 (20, 40, or 80 mg/kg, p.o.); BPH + saw palmetto extract (Saw), TP (3 mg/kg, s.c.), and Saw (100 mg/kg, p.o.); BPH + finasteride (Fi), TP (3 mg/kg, s.c.), and Fi (1 mg/kg, p.o.). Data are expressed as the means ± SEMs (n = 8 per group). ####p < 0.0001, compared with the Con group, ***p < 0.001, ****p < 0.0001 compared with the BPH group. (F) Western blot showing expression of proliferation- and apoptosis-related proteins in BPH-1 cells. Quantitative histogram of protein bands for the expression of (G) Cyclin D1, (H) Cyclin D2, (I) CDK4, (J) p21 and (K) BAX/BCL-2 in BPH-1 cells. BPH- 1 cells were incubated for 6 h in culture medium containing TNF-α (10 ng/ml), INF-γ (10 ng/ml) and HLT-101 (5, 10, or 20) μg/ ml. Data are expressed as the means ± SEMs. #p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001, compared to untreated cells, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared with TNF-α/INF-γ treated cells.

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