Fig. 1.

Fig. 1. Effect of heat-killed Ruminococcus albus (hkRA) on the viability of SH-SY5Y cells. SH-SY5Y cells were treated with various concentrations of hkRA (10², 10³, 10⁴, 10⁵, 10⁶, 10⁷, and 10⁸ cells/ml) for 24 h, and the cytotoxic effect of hkRA was measured using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay (A). SH-SY5Y cells were pretreated with hkRA at concentrations of 10⁶, 10⁷, and 10⁸ cells/ml. After 30 min, the cells were treated with β- amyloid 25–35 (Aβ_25–35) for another 24 h, and cell viability was measured using the MTT assay (B). Data are expressed as the mean ± SD of three independent experiments in triplicate. One-way ANOVA/Tukey’s honestly significant difference analysis was performed. ns; not significant compared with the control.

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