Fig. 4. Effect of LMWCP on hair elongation in a hHF organ culture model.
The hHFs (8 hair follicles/group) were treated with LMWCP (0, 1, and 3 mg/ml) or MNX (50 μM) for 8 days. (A) HFs length was analyzed under a stereomicroscope on days 0, 2, 4, 6, and 8. The relative length of each hair shaft was measured using the ImageJ software. (B) After 10days of culture, the HFs phase was assessed following the hair cycle scoring criteria. Representative images of the HFs for each experimental group are shown, as well as the calculated ratios of the hair cycle phases. (C) H&E staining and IHC staining of β- catenin and VEGF. The results are shown as the mean ± standard deviation. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001 compared to the control group.