Fig. 2.

Fig. 2. Effect of LMWCP on the Wnt-AKT-GSK-3β/β-catenin pathway. (A) hDPCs treated with LMWCP (0, 0.3, 1, and 3 mg/ml) for 1 h were lysed and analyzed for p-AKT(ser⁴⁷³), AKT, p-GSK(Ser⁹), GSK, p-β-catenin(Ser⁶⁷⁵), p-β-catenin (Ser^33/37/Thr⁴¹), β-catenin, PKA, p-PKA (Thr ¹⁹⁷), and β-actin. (B) hDPCs treated with a LMWCP (0, 0.3, 1, and 3 mg/ml) for 24 h were analyzed for Wnt3a, LEF1, β-catenin, VEGF, and β-actin. (C) Expression of β-catenin was analyzed by ICC. Representative data from three independent experiments are shown. The results are shown as the mean ± standard deviation. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001 compared to the control group.

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