Fig. 1.

Fig. 1. Effect of the LMWCP on proliferation and cellular energy metabolism in hDPCs. (A) Proliferation of hDPCs was assessed after LMWCP treatment (0, 0.1, 0.3, 1, and 3 mg/ml) for 24 h. (B) The expression of PCNA after treatment with a LMWCP for 24 h. (C) JC-1 aggregates (A590)/monomer (A530) ratio of DPCs treated with LMWCP (0, 1, and 3 mg/ml) and MNX (1 μM) for 24 h. (D) JC-1 monomer form (green) and aggregate form (red) were detected using fluorescent microscopy. (E) The expression of cyclin D1, cyclin E, CDK2, and CDK6 after treatment with LMWCP for 24 h. (E) Cultured media from hDPCs treated with either vehicle, growth media, or LMWCP (0, 0.3, 1, and 3 mg/ml) for 48 h was used for analysis using the growth factor antibody array. (F) The mRNA expression levels of EGF, HB-EGF, FGF-4, and FGF-6 in hDPCs treated with LMWCP (3 mg/ml) for 1 h were analyzed by qPCR (n = 3). The results are shown as the mean ± standard deviation. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001 compared to the control group.

© J. Microbiol. Biotechnol.