Fig. 4.

Fig. 4. Alteration of the gut microbiota by heme-SCP. The gut microbiota of mice fed with heme-SCP (0, 0.05, and 0.5%) was analyzed. The feces from five mice of each group were combined for 16S rRNA sequencing. (A) Relative abundance plots of top 40 genera were displayed using EzBioClould database. (B) Alpha diversity was predicted using the numbers of observed operational taxonomic unit (OTU) (left) and Shannon indices (right). (C) Beta diversity comparison of fecal bacterial communities obtained from heme-SCP-fed mice between 10 and 28 days. The two-dimensional principal coordinate analysis (PCoA) based on Bray-Curtis dissimilarity metrics is displayed. (D) Heatmap of anti-obesity biomarkers was generated using the numbers of16S rRNA amplicons. Differences of relative abundance in bacterial genera between heme-SCP (0.05 or 0.5%) diet and no treatment is indicated in blue (decrease) or red (increase). Gray indicates ‘not determined’.

© J. Microbiol. Biotechnol.