Fig. 3. Diagnostic ability of typhoidal Salmonella heptaplex PCR on recovered Salmonella colonies from 6-h cultured blood samples inoculated with typhoidal Salmonella serovars.
Panel A: DNA extraction using UltraPrepMan solution, Panel B: DNA extraction using TE buffer (pH 8.0), Panel C: PCR on colony. Lane 1: Salmonella Typhi; lane 2: S. Paratyphi A; lane 3: S. Paratyphi B, lane 4; S. Paratyphi C; lane M: DNA marker; P: positive control with genomic DNA of Salmonella Typhi; NT: No template DNA.