Journal of Microbiology and Biotechnology

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Table. 2. Table 2

Primer pairs used for typhoidal Salmonella heptaplex PCR and their expected result with typhoidal Salmonella serovars.

Target gene (synonym)	Primer	Primer sequence (5' --- 3')	Primer concentration (μmol/l)	PCR product size (bp)	Target Salmonella serovars or subspecies	Expected results with typhoidal Salmonella serovars^a				References
Target gene (synonym)	Primer	Primer sequence (5' --- 3')	Primer concentration (μmol/l)	PCR product size (bp)	Target Salmonella serovars or subspecies	Typhi	Paratyphi A	Paratyphi B	Paratyphi C	References
STM3098	STM3098 -F3	TTTRG CGGCR CAGGC GATTC	1	423	Genus Salmonella	+	+	+	+	[24,26]
	STM3098 -R3	GCCTC CGCCT CATCA ATYCG
STY4578	STY4578 F32	CATTTCTGAGATTTAT TCTGACGCTTGTG	0.5	291	Typhi, Paratyphi C	+	-	-	+	In this study
	STY4578 R322	CTGAATATTCGCAAA TCGCGACG
STY1599	STY1599 F	TTACC CCACA GGAAG CACGC	0.15	258	Typhi	+	-	-	-	[26]
	STY1599 R2	CTCGT TCTCT GCCGT GTGGG
STY3279	STY 3279 F102	AATCA GCAGT GCGTT GAGAA AACC	1	193	Typhi, Paratyphi A	+	+	-	-	In this study
	STY3279 R294	GGAGT TAATA AGTGA TAGGA ACATT GTACT TACTG T
STY3670	STY3670 47F	CCTTGGCTGGATGTG CTTTG	0.75	165	Typhi, Paratyphi B	+	-	+	-	In this study
	STY3670 211R	AGCCAGGAACTTCGT CACTC
STM4057	STM4057 F3	GGTGG CCTCS ATGAT TCCCG	0.375	137	Salmonella subspecies enterica (I)	+	+	+	+	[24,26]
	STM4057 R	CCCAC TTGTA GCGAG CGCCG
STY2750	STY2750 F7	TTTCT GTGTA GYGCA CAGCT TCTGG C	0.75	70	Typhi, Paratyphi A	+	+	-	-	In this study
	STY2750 R76	TGCTG CCAGT GAAAC CCACT ATTGT GTCG
IAC^b	-	-	100			+	+	+	+

^a+, Positive result; -, Negative result

^bIAC, Internal amplification control in heptaplex PCR

J. Microbiol. Biotechnol. 2023;33:1457~1466 https://doi.org/10.4014/jmb.2307.07031