Fig. 1.

Fig. 1. Performance of typhoidal Salmonella heptaplex PCR for detecting typhoidal Salmonella serovars. Panel A: Heptaplex PCR results with genomic DNAs from various Salmonella strains electrophoresed on a 3.5% agarose gel at 100 V for 70 min. M, DNA ladder; lane 1~5 Salmonella Typhi ATCC 33459, NCCP 14641, NCCP 10820, NCCP 12201, NCCP 10340; lane 6-12, S. Paratyphi A NCCP 14759, S11 (food isolate), 12-01 (clinical isolate), 12-02 (clinical isolate), 12-05 (clinical isolate), 12-07 (clinical isolate), 13-02 (clinical isolate); lane 13-14, S. Paratyphi B ATCC 10719, NCCP 12204; lane 15, S. Paratyphi C ATCC 13428; lane 16-18, S. Typhimurium ATCC 19585, ATCC 13311, ATCC 14028; lane 19, S. choleraesuis ATCC 13312; lane 20, S. Enteritidis ATCC 4931, lane 21, S. gallinurum ATCC 9184; lane 22, S. pullorum ATCC 9120; lane 23, S. subspecies salamae ATCC 15793; lane 24, S. subspecies arizonae ATCC 13314; lane 25, S. subspecies diarizonae ATCC 43973; lane 26, S. subspecies houtenae ATCC 43974; lane 27, S. enterica subspecies indica ATCC 43976; lane 28, S. bongori ATCC 43975, lane 29, no template DNA. Panel B: Analysis of heptaplex PCR results for Salmonella Typhi, Paratyphi A, Paratyphi B, and Paratyphi C using an Agilent Bioanalyzer 2100. The X-axis on the electropherogram represents the amplicon size (bp) and the Y-axis represents the fluorescence units (FUs). The arrow(s) showed the specific amplification marker(s) of each Salmonella serovar.

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