Journal of Microbiology and Biotechnology

eISSN 1738-8872
pISSN 1017-7825

Table. 1.

Table. 1. Table 1

Overview of estimates of read sequence diversity and phylotype coverage of NovaSeq data generated from larva and adult bee samples.

Group^a	Analyzed reads	OTU^b	Chao1	Shannon	Simpson^c	Good’s coverage	Bacterial abundance^d
L1	1049	306	889.03	4.65	14.88	0.80	425.70±4.32
L2	984	295	893.78	4.54	12.84	0.79	1397.20±3.55
NG1	1479	343	1096.38	4.28	12.62	0.83	5170.29±52.52
NG2	1516	336	1372.03	4.10	10.76	0.83	29,875.68±151.76
NG3	1829	370	1425.68	4.09	11.30	0.85	45,940.38±700.02
NG4	1750	333	1134.24	4.06	12.69	0.86	21,011.64±106.73
NG5	2689	957	2046.66	6.20	76.81	0.78	34,351.13±959.45
NG6	1494	407	1304.06	4.74	19.81	0.80	38,147.12±96.89
NG7	1663	337	1294.03	4.03	12.36	0.84	42,258.16±536.61
NG8	1596	438	1589.63	4.66	16.16	0.79	63,283.78±3372.10
NG9	1396	276	979.64	3.76	9.32	0.85	78,337.94±3181.66
VG1	1078	316	951.63	4.72	20.48	0.79	28,860.83±1318.50
VG2	1620	350	1476.44	4.07	11.61	0.83	28,369.44±1008.31
VG3	1567	419	1634.24	4.52	13.50	0.80	60,108.58±1526.31
VG4	1723	301	1177.72	3.63	7.89	0.87	45,362.68±1727.35
VG5	1681	332	1131.03	4.10	11.09	0.86	9437.09±95.87

The diversity indices and richness estimators were calculated using Mothur software. Diversity was estimated using operational taxonomic units (OTUs) and was defined as groups with ≥97% sequence similarity.

^aL, NG, and VG denote the larval, non-Varroa, and Varroa groups, respectively.

^bThe OTUs were determined based on 97% of 16S rRNA gene similarity.

^cInverse-Simpson (see the materials and methods)

^d16S rRNA gene copies per ng (gDNA) estimated by qPCR, and data are means ± standard deviation from triplicate reactions (see Materials and Methods).

J. Microbiol. Biotechnol. 2023;33:1495~1505 https://doi.org/10.4014/jmb.2306.06040