Fig. 4.

Fig. 4. Analysis of multilayer coating effect on LAB strains adhesion and proliferation in hPSC-derived hIECs. (A) Representative images of MUC13 expression in hIECs. Scale bar: 100 μm. (B) TEER values of hIECs (n = 3). (C-E) Analysis of adhesion ability in relation to the initial number of (C) LF, (D) RE, and (E) LC, at adhesion (2 h) and proliferation (24 h) in hIECs (n = 4). (F-H) Representative fluorescence images of (F) LF, (G) RE, and (H) LC at adhesion (2 h) and proliferation (24 h) in hIECs. Green fluorescence indicates bacterial peptidoglycan. Scale bar: 20 μm. (I) Analysis of adhesion (2 h) ability and proliferation (24 h) of LF in hIECs, after passing through gastric juice. The two groups were compared by a two-tailed t-test (n = 4; *p < 0.05, **p < 0.01 and ***p < 0.001).

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