Fig. 4.

Fig. 4. In vitro processing of fusion proteins containing engineered linkers. SDS-PAGE analysis of culture supernatant of (A) HL-CbPhy, (B) HL-EXD4, and (C) HL-ZmBD2, with and without Kex2p digestion. Structure of fusion proteins are indicated. C: proteins expressed without HL fusion partner. The corresponding protein bands are indicated by arrows. M: protein molecular weight markers. (D) Amino acid sequence and ProP score of the modified linkers.

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