Fig. 3.

Fig. 3. Production of HL-EGF variants by fed-fermentation and IMAC purification. SDS-PAGE analysis of culture broth (10 μl) of strains expressing (A) HK2-EGF, (B) HK3-EGF, and (C) HK4-EGF at the indicated times. Structure of fusion proteins are indicated. HL-EGF and EGF bands are indicated by arrows. (D) Purification of HK2-EGF by IMAC. lane 1: eluted fraction from 1^st IMAC, lane 2: after digestion with Kex2p, lane 3: unbound fraction from 2^nd IMAC. M: protein molecular weight markers. (E) Bioactivity assay of the purified hEGF.

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