Fig. 2.

Fig. 2. P₃ and P₄ site engineering for in vitro processing of HL-hEGF fusion proteins. (A) Schematic diagram of the MFα-HL18-hEGF fusion protein. (B) Amino acid sequences and ProP scores of modified linkers. (C) SDS-PAGE analysis of concentrated culture supernatants of transformants expressing YEGα-HL18-EGF (lane 1), YEGα-HK1-EGF (lane 2), YEGα-HK2-EGF (lane 3), YEGα-HK3-EGF (lane 4), YEGα-HK4-EGF (lane 5), concentrated culture supernatants of transformants expressing YEGα-HL18-EGF after digestion with EK (lane 6), concentrated culture supernatants of transformants expressing YEGα-HK1-EGF (lane 7), YEGα-HK2-EGF (lane 8), YEGα-HK3-EGF (lane 9), and YEGα-HK4-EGF (lane 10) after digestion with Kex2p. M: protein molecular weight marker.

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