Purification table for hEGF.
Procedure | HK2-hEGF | HK3-hEGF | HK4-hEGF | |||
---|---|---|---|---|---|---|
Total protein (mg) | Yield (%) | Total protein (mg) | Yield (%) | Total protein (mg) | Yield (%) | |
Fermentation sup | 930 | 100 | 858 | 100 | 650 | 100 |
1st IMAC | 407 | 44 | 211 | 24 | 124 | 19 |
2nd IMAC | 162 | 18 | 76 | 9 | 40 | 6 |