Fig. 1.

Fig. 1. P₁' and P₂' site engineering to increase in vivo processing of MFα propeptide. (A) Schematic diagram of YEGα-HL28-EGF and YEGα-HL18-EGF vector. The amino acid sequence of HL peptide are indicated. (B) SDS-PAGE analysis of the culture supernatant expressing YEGα-HL28-EGF (lanes 1–4) and YEGα-HL18-EGF (lanes 5–8). Concentrated culture supernatants (lanes 1 and 5). Deglycosylated culture supernatants (lanes 2 and 6). Culture supernatants treated with Kex2p (lanes 3 and 7). Culture supernatants treated with EK (lanes 4 and 8). M: protein molecular weight marker.

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