Fig. 7.

Fig. 7. TLR9 regulates the pathogenesis of FGR through the gut microbiota. (A) The litter size, pregnancy weight, and fetal weight were determined. (B) ELISA was performed to examine the contents of IL-1β, TNF-α, IL-10, LPS and LBP in maternal rats' serum; (C) ELISA was applied to measure relative inflammatory marker levels in cord blood; (D) IHC staining was performed to assess the expression of TLR9 in placental tissue; (E) The abundance of relative proteins was shown by Western blot; (F) The effects of ODN1668 and HCQ on TRAF3, TBK1, IRF3, and NF-κBp65 expression in placental tissue were determined by Western blot; (G) The number of CD11b-positive cells was assessed by flow cytometry; (H) The abundance of PCNA, Ki67, and MMP9 was assessed by Western blot. *p < 0.05 compared with the FGR group. &p < 0.05 compared with the HCQ group. # p < 0.05 compared with the FMT group.

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