JMB

eISSN 1738-8872
pISSN 1017-7825

Fig. 2.

Download original image
Fig. 2. Genetically encoded biosensor-based directed evolution. (A) Directed evolution of enzymes with the assistance of FACS and genetically encoded biosensors. (B) Library construction and high-throughput screening to direct the evolution of the metabolic pathway with the assistance of biosensors. (C) Whole genome-directed evolution with the assistance of whole-cell biosensors in multi-well plates to avoid the accumulation of mutations in biosensors. (D) Continuous directed evolution with simultaneous mutation and selection in the cell growth process. Higher IPP production would generate lower expression levels of MutD5 and RFP (red fluorescence protein), resulting in the accumulation of positive mutations after screening the low red fluorescent cells.
J. Microbiol. Biotechnol. 2023;33:1257~1267 https://doi.org/10.4014/jmb.2304.04031
© J. Microbiol. Biotechnol.