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Fig. 1.

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Fig. 1. Mechanisms and engineering strategies of the genetically encoded biosensor. (A) The structure and signal transduction mechanism of TCBs. (B) Engineering strategies for changing the specificity and inducer dose curve of TCBs by domain swapping and expression level optimization. (C) Engineering strategies of TFBs. LBD or DBD engineering is often performed to change biosensor specificity, while promoter engineering is performed to regulate the expression level of TFs and reporter genes, which could manipulate the dynamic range, sensitivity, and leakage expression level of TFBs. (D) The mechanism of RBS-based riboswitch and ribozyme-based riboswitch.
J. Microbiol. Biotechnol. 2023;33:1257~1267 https://doi.org/10.4014/jmb.2304.04031
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