Fig. 5.

Fig. 5. The effect of kinetin riboside on colorectal cancer cell growth. (A) SW480 and HCT116 cells were treated with kinetin riboside (10, 20, or 40 μM) for 48 h, and cell viability was measured using a CellTiter-Glo assay. (B) SW480 and HCT116 cells were incubated with vehicle (DMSO) or kinetin riboside (10, 20, or 40 μM) for 48 h and then stained with Annexin V-FITC and propidium iodide (PI). The x- and y-axes indicate annexin V-FITC intensity and PI fluorescence, respectively. (C) SW480 and HCT116 cells were incubated with vehicle (DMSO) or kinetin riboside (10, 20, or 40 μM) for 48 h, and whole-cell extracts were analyzed by Western blotting with anti-caspase-3, anti-cleaved caspase-3, and anti-poly (ADPriboside) polymerase (PARP).

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