Fig. 4.

Fig. 4. Kinetin riboside decreases the levels of β-catenin and its target genes in colorectal cancer cells. (A, B) SW480 and HCT116 cells were incubated with vehicle (DMSO) or kinetin riboside (10, 20, or 40 μM) for 15 h, and cytosolic proteins were analyzed by Western blotting with anti-β-catenin antibody. (C, D) Whole-cell extracts were prepared from SW480 and HCT116 cells incubated for 48 h, and proteins were analyzed by Western blotting with anti-cyclin D1 and anti-c- Myc antibodies. In (A) to (D), β-actin was used as a loading control, and β-catenin levels were normalized to those of β-actin. The bar graph indicates the average volume density corrected for the loading control, and results are expressed as the mean ± SD of three independent experiments. *p < 0.05 and **p < 0.01, comparison between the DMSO control and kinetin ribosidetreated groups.

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