JMB

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Table. 1.

Table. 1.

Primers used for the LAMP assay.

Primer name Tm (°C) GC rate Sequence (5′- 3′) Target gene
SMspaP-F3 54.5 55.6% TTAGGCAGGGTCAAGGTG spaP
SMspaP-B3 51.1 42.9% CAATTGCCATTGTAGGATCAG spaP
SMspaP-FIP (F1c-F2) 69.0 50.0% GAGTTAGGCGCATCTGAACTATCCGGACCATGTATACCAGAGCT spaP
SMspaP-BIP (B1c-B2) 68.3 48.8% GGTATGGTGCTGGTGCTATCCACAAACAAGTGTTGATGAGA spaP
SMspaP-LoopF 58.1 64.7% CCAGATCCCGGTTCGCT spaP
SMspaP-LoopB 54.8 45.8% GCTTGGTCCTAATAACAGTGTGAC spaP

Tm, melting temperature

J. Microbiol. Biotechnol. 2023;33:1101~1110 https://doi.org/10.4014/jmb.2304.04026
© J. Microbiol. Biotechnol.