Primers used for the LAMP assay.
Primer name | Tm (°C) | GC rate | Sequence (5′- 3′) | Target gene |
---|---|---|---|---|
SMspaP-F3 | 54.5 | 55.6% | TTAGGCAGGGTCAAGGTG | |
SMspaP-B3 | 51.1 | 42.9% | CAATTGCCATTGTAGGATCAG | |
SMspaP-FIP (F1c-F2) | 69.0 | 50.0% | GAGTTAGGCGCATCTGAACTATCCGGACCATGTATACCAGAGCT | |
SMspaP-BIP (B1c-B2) | 68.3 | 48.8% | GGTATGGTGCTGGTGCTATCCACAAACAAGTGTTGATGAGA | |
SMspaP-LoopF | 58.1 | 64.7% | CCAGATCCCGGTTCGCT | |
SMspaP-LoopB | 54.8 | 45.8% | GCTTGGTCCTAATAACAGTGTGAC |
Tm, melting temperature