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Fig. 4.

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Fig. 4. mRNA and protein expression levels of muscle markers in C2C12 myotubes. (A, B) The expression of Atrogin-1 and MuRF-1 (muscle atrophy-related markers) and MyoD (differentiation marker) in C2C12 myotubes were measured via RT-qPCR. The mRNA expression level was expressed as a ratio after normalization with the levels of β-actin. (C) Representative images of western blots for MuRF-1 protein extracted from C2C12 myotubes. The MuRF-1 band can be observed at the 40-kDa marker position. The relative ratio of protein/β-actin protein is represented as the fold change of the DIF control group (D). Data are expressed as the mean ± SD. *p < 0.01 vs. DIF control, #p < 0.01 vs. DIF + DEX control.
J. Microbiol. Biotechnol. 2023;33:915~925 https://doi.org/10.4014/jmb.2303.03001
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