Fig. 5. Combination of UV and sodium nitrite mutagenesis for screening of tylosin high-production mutants.A: Preliminary screening of mutant strains in 24-well plates, the last column is wild-type strain SF-3. B-F: Screening of high-yield strains in shake flasks. In each screening experiment, the wild-type strain SF-3 was used as the control. Data are shown as mean ± SD from three independent replicates.