Fig. 4.

Fig. 4. Sodium nitrite mutagenesis for screening of tylosin high-production mutants. A: Lethal curve of sodium nitrite mutagenesis. B: Positive mutation rate of sodium nitrite mutagenesis. C: Preliminary screening of high-yield strains by sodium nitrite in 24-well plates, the last column is wild-type strain SF-3. D-G: Rescreening of high-yield strains in shake flasks. In each screening experiment, the wild-type strain SF-3 was used as the control. Data are shown as mean ± SD from three independent replicates.

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