Fig. 3.

Fig. 3. UV mutagenesis for screening of tylosin high-yielding mutants. A: Lethality rate of spore suspensions after UV mutagenesis. B: Positive mutation rate by UV mutagenesis. C: Preliminary screening of high-yield strains detected by UV spectrophotometry after growth in 24-well plates, the last column is wild-type strain SF-3. D: Re-screening of high-yield strains detected by HPLC after shake flask culture. In each screening experiment, the wild-type strain SF-3 was used as the control. Data are shown as mean ± SD from three independent replicates.

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