Fig. 5.

Fig. 5. Fisetin ameliorated H₂O₂-induced cell cycle arrest, apoptosis and mitochondria impairment in C2C12 cells. Cells were cultured for 1 hour in medium containing fisetin and ZnPP or not, and then treated with H₂O₂ for an additional 24 h. Cell cycle distribution, induction of apoptosis and changes in MMP were evaluated by flow cytometry. (A and B) The frequencies of cells belonging to each stage of the cell cycle (A) and the sub-G1 phase, which is the apoptosis index, were shown (B). (C and D) After staining with annexin V/PI, flow cytometry was performed, and representative histograms (C) and the results of quantitative analysis (D) were shown. (E) Cell viability of cells cultured under the same conditions was assessed by the MTT assay. (F and G) After JC-1 staining, representative flow cytometry histograms were indicated (F), and the ratio of JC- 1 monomers in cells in each treatment group was expressed as mean ± SD (G). ***p < 0.001 vs. control group; ^##p < 0.01 and ^###p < 0.001 vs. H₂O₂-treated cells; ^$p < 0.05, ^$$p < 0.01 and ^$$$p < 0.001 vs. fisetin + H₂O₂ treatment group.

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