Fig. 3.

Fig. 3. Fisetin alleviated DNA damage in H₂O₂-treated C2C12 cells. Cells exposed with or without fisetin and NAC for 1 h were stimulated with H₂O₂ for another 24 h. (A) Representative immunofluorescence images following comet assay were indicated. Scale bar is 250 μm. (B) Result of DNA damage score using OpenComet software. Data indicate mean ± SD values (n = 3; ***p < 0.001 vs. control cells; ^###p < 0.001 vs. H₂O₂‐treated cells). (C) After treatment, contents of 8- OHdG, which is the deoxyriboside form of 8-oxoGuanine, were measured. (D) After performing fluorescence staining to evaluate the expression of γH2AX (red), the nuclei were further stained with DAPI (blue) and visualized with a fluorescence microscope. Scale bar is 100 μm.

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