Fig. 2.

Fig. 2. Fisetin attenuated ROS production in H₂O₂-treated C2C12 cells. Cells exposed with or without fisetin and NAC for 1 h were stimulated with H₂O₂ for another 1 h. The level of ROS production was investigated by performing DCF-DA staining. (A and B) Representative flow cytometry histograms (A) and mean values of the data were presented (B). ***p < 0.001 vs. control group; ^###p < 0.001 vs. H₂O₂-treated cells. (C) Representative immunofluorescence images following DCF-DA staining were indicated. Scale bar is 30 μm.

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