Fig. 1.

Fig. 1. Fisetin inhibited the reduction of cell viability caused by H₂O₂ treatment in C2C12 cells. (A-C) MTT assay was performed after cells were treated with various concentrations of fisetin and H₂O₂ for 24 h (A and B) or pretreated with or without fisetin and NAC for 1 h followed by stimulation with H₂O₂ for an additional 24 h (C). *p < 0.05, **p < 0.01 and ***p < 0.001 vs. control group; ^##p < 0.01 and ^###p < 0.001 vs. H₂O₂-treated cells. (D) Representative morphological images of cells exposed to H₂O₂ in the presence or absence of fisetin were presented (200x). Scale bar is 50 μm.

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