Fig. 8. Production of ABA metabolites by CaCYP707A2 from soluble fraction over time.
CaCYP707A2 protein from soluble fraction was incubated with (+)-ABA and CaCPR for indicated time (15-120 min). Production of ABA metabolite over time was calculated from area on chromatogram of HPLC results (A). The PA generation ratio over time was calculated from the product amount of 8'-hydroxy ABA plus PA (B). The values are represented as the mean with SD of triple measurements. 8’-OH: 8’-hydroxy ABA, PA: phaseic acid.