Fig. 7.

Fig. 7. HPLC analysis of ABA metabolites produced by CaCYP707A2. CaCYP707A2 protein from membrane fraction was incubated with (+)-ABA and CaCPR (A), rat CPR (B), and FdR/Fdx (C) in the absence (left; –NGS) or presence (right; +NGS) of a NADPH-generating system. Metabolites produced by CaCYP707A2 catalytic activity were analyzed by HPLC. The peaks marked by red and blue arrows are 8’-hydroxy ABA (8’-OH) and phaseic acid (PA), respectively, whereas the peaks marked by black arrows are abscisic acid (ABA).

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