Fig. 6.

Fig. 6. Binding titration of CaCYP707A2 with ABA. (A) The 0.5μM CaCYP707A2 was divided into each of two glass cuvettes and baseline was set. (+)-ABA was added to the cuvette at ranging from 0 to 80 μM, and difference spectra was recorded between 500 nm and 350 nm. With increasing concentrations of (+)-ABA, spectra were increased at 386 nm and decreased at 419 nm. (B) The difference between absorbance at 389 nm and 420 mm were recorded using the dual wavelength mode, as a function of (+)-ABA.

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