eISSN 1738-8872
pISSN 1017-7825
pISSN 1017-7825
Table. 2.
Table. 2.
Primers and PCR conditions for antimicrobial resistance genes tested in this study.
| Resistance gene | Primers | Primer sequence (5′-> 3’) | Amplicon size (bp) | Reference(s) | |
|---|---|---|---|---|---|
| aadE-1 | GCAGAACAGGATGAACGTATTCG | 55 | 369 | [17]. | |
| aadE-2 | ATCAGTCGGAACTATGTCCC | ||||
| blaZ-1 | CAGTTCACATGCCAAAGAG | 52 | 846 | [18]. | |
| blaZ-2 | TACACTCTTGGCGGTTTC | ||||
| cat | cat-1 | TTAGGTTATTGGGATAAGTTA | 44 | 300 | [19]. |
| cat-2 | GCATGRTAACCATCACAWAC | ||||
| ermA-1 | AAGCGGTAAACCCCTCTGA | 55 | 190 | [20]. | |
| ermA-2 | TTCGCAAATCCCTTCTCAAC | ||||
| ermB-1 | TTTTGAAAGCCGTGCGTCTG | 55 | 202 | [17] | |
| ermB-2 | CTGTGGTATGGCGGGTAAGTT | ||||
| ermC-1 | AATCGTCAATTCCTGCATGT | 55 | 299 | [20] | |
| ermC-2 | TAATCGTGGAATACGGGTTTG | ||||
| lnuA-1 | GGTGGCTGGGGGGTAGATGTATTAACTGG | 56 | 323 | [21] | |
| lnuA-2 | GCTTCTTTTGAAATACATGGTATTTTTCGATC | ||||
| tetK-1 | CAATACCTACGATATCTA | 50 | 352 | [17] | |
| tetK-2 | TTGAGCTGTCTTGGTTCA | ||||
| tetL-1 | TGGTCCTATCTTCTACTCATTC | 53 | 385 | [22] | |
| tetL-2 | TTCCGATTTCGGCAGTAC | ||||
| tetM-1 | TCAACACATCGAGGTCCGTC | 58 | 737 | this study | |
| tetM-2 | TCGCAACCATAGCGTATCCC | ||||
| tetO-1 | AGCGTCAAAGGGGAATCACTATCC | 55 | 1723 | [17] | |
| tetO-2 | CGGCGGGGTTGGCAAATA | ||||
| TetB-1 | AAAACTTATTATATTATAGTG | 46 | 169 | [23] | |
| TetB-2 | TGGAGTATCAATAATATTCAC | ||||
| TetQ-1 | AGAATCTGCTGTTTGCCAGTG | 63 | 169 | [23] | |
| TetQ-2 | CGGAGTGTCAATGATATTGCA | ||||
| tetS-1 | ATCAAGATATTAAGGAC | 55 | 573 | [24. 25] | |
| tetS-2 | TTCTCTATGTGGTAATC′ | ||||
| TetT-1 | AAGGTTTATTATATAAAAGTG | 46 | 169 | [23] | |
| TetT-2 | AGGTGTATCTATGATATTTAC | ||||
| tetW-1 | ATATTGGAATTCTTGCCCAT | 48 | 510 | this study | |
| tetW-2 | ATGCTTCTATGTCGGTATTT | ||||
| tetMgr-1 | GAYACICCIGGICAYRTIGAYTT | 45 | 1100 | [26]. | |
| tetMgr-2 | GCCCARWAIGGRTTIGGIGGIACYTC |
J. Microbiol. Biotechnol. 2023;33:500~510 https://doi.org/10.4014/jmb.2210.10041
© J. Microbiol. Biotechnol.