Fig. 5.

Fig. 5. LINC01232 overexpression facilitated cell proliferation and angiogenesis by decreasing miR-181a-5p level. (A-B). The transfection efficiency of miR-181a-5p mimic and miR-181a-5p inhibitor was detected by qRT-PCR. U6 acted as an internal reference. (C-D). CCK-8 assay was carried out to examine the proliferation of SW-620 and LOVO cells transfected or untransfected with siLINC01232, LINC01232 overexpression plasmid, miR-181a-5p mimic and miR-181a-5p inhibitor. (E-H). Tubule formation assay was applied to determine the angiogenesis of SW-620 and LOVO cells transfected or untransfected with siLINC01232, LINC01232 overexpression plasmid, miR-181a-5p mimic and miR-181a-5p inhibitor. ^*vs. IC+siNC, ^vs. I+siNC, ^#vs. IC+siLINC01232, ^&vs. MC+NC, ^Δ vs. M+NC, ^†vs. MC+LINC01232; ^*or^or^#or^&or^† p < 0.05, **or ^ΔΔ or^†† or^&& p < 0.01, ^***or^^^ or ^### or^&&& or ^ΔΔΔ or ^†††p < 0.001. qRT-PCR, quantitative reverse transcription polymerase chain reaction; CCK-8, cell counting kit-8; I, miR-181a-5p inhibitor; IC, inhibitor control; M, miR-181a-5p mimic; MC, mimic control; siNC, siRNA negative control.

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