The primer sequences and predicted sizes used in the PCR.
Target gene | Oligonucleotide sequence (5'-3') | Amplicon size (bp) |
---|---|---|
16S rDNA | 16Sf: CAG CTC GTG TCG TGA GAT GT | 420 |
16Sr: AAT CAT TTG TCC CAC CTT CG | ||
sauf: AATCTTTGTCGGTACACGATATTCTTCACG | 107 | |
saur: CGTAATGAGATTTCAGTAGATAATACAACA | ||
mecAf: AAA ATC GAT GGT AAA GGT TGG C | 532 | |
mecAr : AGT TCT GCA GTA CCG GAT TTG C | ||
aacA-aphDf: TAA TCC AAG AGC AAT AAG GGC | 227 | |
aacA-aphDfr: GCC ACA CTA TCA TAA CCA CTA | ||
tetKf: GTA GCG ACA ATA GGT AAT AGT | 360 | |
tetKr: GTA GTG ACA ATA AAC CTC CTA | ||
tetMf: AGT GGA GCG ATT ACA GAA | 158 | |
tetMr: CAT ATG TCC TGG CGT GTC TA | ||
vatAf: TGG TCC CGG AAC AAC ATT TAT | 268 | |
vatAr: TCC ACC GAC AAT AGA ATA GGG | ||
vatBf: GCT GCG AAT TCA GTT GTT ACA | 136 | |
vatBr: CTG ACC AAT CCC ACC ATT TTA | ||
vatCf: AAG GCC CCA ATC CAG AAG AA | 467 | |
vatCr: TCA ACG TTC TTT GTC ACA ACC | ||
ermAf: AAG CGG TAA ACC CCT CTG A | 190 | |
ermAr: TTC GCA AAT CCC TTC TCA AC | ||
ermCf: AAT CGT CAA TTC CTG CAT GT | 299 | |
ermCr: TAA TCG TGG AAT ACG GGT TTG |