Fig. 3.

Fig. 3. Inhibitory effects of SFEN on the transcription activity of NF-κB and the upstream regulator proteins of NF-κB. (A)Transcription activity of NF-κB was measured by luciferase reporter gene assay described in Materials and Methods. Bars marked with different letters (a–c) are significantly different (p < 0.05) according to Tukey's test. The hashes (#) indicate a significant difference (p < 0.05) compared to untreated control. (B) Phosphorylated and total forms of IKKα/β (Ser176/180) and IκBα (Ser32) proteins were determined by western blot assay as described in Materials and Methods.

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