Fig. 5.

Fig. 5. Identification of a novel antigen recognized by the P1 Ab. (A) The P1 Ab recognized human POSTN protein and murine bone marrow lysates when analyzed by western blotting. (B) Beta-like cell gene expression as determined by RNA sequencing analysis. Highly expressed beta cell markers from the RNAseq analysis (n = 3) are summarized as Transcripts Per Million mapped reads (TPM). (C) Human CD34⁺ cells were treated with P1 Ab or control isotype antibody, and cell lysates were assessed by western blotting using antibodies against phosphorylated (p-) AKT and ERK.

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