Fig. 4.

Fig. 4. An agonist antibody induces beta-like cell differentiation. (A) Beta-like cells induced by the P1 Ab. Human CD34⁺ cells were incubated with P1 Ab (10 μg/ml) for 2 weeks. The red arrow in the image on the left indicates beta-like cells, which are magnified in the image to the right. (B) Insulin levels in the culture media were measured, and treatment with P1 Ab increased levels of insulin. . ***p-value, 0.0005; Student’s t-test. (C) Human CD34⁺ hematopoietic stem cells were incubated with P1 antibody and harvested islets were analyzed by flow cytometry. Cells were then stained with anti-Insulin, PDX-1, and Nkx-6.1. Scale bars = 50 μm.

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