Fig. 3.

Fig. 3. Antibody induced differentiation of bone marrow cells into beta-like cells. P1 Ab (50 μg/mouse, i.p., two times/week) was injected into lethally irradiated wild-type mice. After 3 weeks, the mice were perfused with PBS prior to harvesting the islets for immuno-fluorescence histochemistry. Islets were stained with DAPI, anti-Insulin, PDX-1 (beta cell marker), and glucagon (alpha cell marker). (A) Significantly more Insulin⁺ and PDX-1⁺ staining was identified in the P1 Abtreated dTomato⁺ tissues as compared to controls, suggesting that the beta-like cells migrated from the bone marrow to the islet. (B) Glucagon was not detected on the dTomato⁺ Insulin⁺ cells. Scale bars = 50 μm.

© J. Microbiol. Biotechnol.