Fig. 1.

Fig. 1. Scheme for the in vivo phenotype selection of the P1 antibody that induces differentiation and migration of mouse HSCs. Genes from a human ScFv phage library (10⁸ members) were cloned into a lentiviral vector to make a lentiviral membrane-tethered library in which antibody molecules are attached to the plasma membrane and displayed on the cell surface. Total mouse bone marrow cells were infected with the antibody library in vitro and transplanted into lethally irradiated C57BL/6J mice. The system is autocrine based because each cell has a different antibody and the putative target. After 7 days, the mouse pancreatic tissues were harvested and analyzed by PCR to identify any antibody genes in cells that traffic to the islet.

© J. Microbiol. Biotechnol.