JMB

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Table. 4.

Table. 4.

Different extraction methods for polysaccharides from yeast cell walls.

Extraction methods Advantage Disadvantage
Alkaline extraction Short extraction time; low extraction cost; high product purity The operation is cumbersome and requires strict control of the lye concentration and reaction time
Enzyme extraction Simple operation; under the action of multiple enzymes, impurities such as chitin are completely removed, reducing the difficulty of subsequent separation Multiple enzymes are required to work together and the enzymatic hydrolysis takes a long time (about 12 h)
Ultrasonic extraction Low extraction temperature; short extraction time; convenient for subsequent product purification; no effect on the structure and physicochemical properties of the polysaccharides The operation is complicated, and the extraction conditions need to be explored; when the temperature is too high, the properties of the polysaccharides will be destroyed; small processing capacity
Microwave extraction High purity of extracted product; less waste is produced; mild reaction conditions The operating conditions are strict, and the extraction temperature needs to be strictly controlled; the extraction cost is high; the processing volume is small, which is not suitable for mass production
J. Microbiol. Biotechnol. 2023;33:151~166 https://doi.org/10.4014/jmb.2207.07057
© J. Microbiol. Biotechnol.