Fig. 2.

Fig. 2. p170 and p170-RBD could form a dimer structure and assembled into VLPs. (A) and (C) SDS–PAGE of p170 and p170-RBD, respectively. Lane 1, reducing SDS–PAGE for p170 and p170-RBD; Lane 2, non-reducing SDS–PAGE for p170 and p170-RBD. The dimer in non-reducing SDS–PAGE was twice the size of the monomer in reducing SDS–PAGE. (B) and (D) Western blot of p170 and p170-RBD, respectively, with an anti-His monoclonal antibody. Lane 1, reducing SDS–PAGE for p170 and p170-RBD; Lane 2, non-reducing SDS–PAGE for p170 and p170-RBD. (E) and (F) Nanoparticle size analysis of p170 and p170-RBD, respectively. The diameter of the p170 particles was approximately 18 nm and that of p170-RBD was approximately 24 nm. (G) and (H) TEM analysis of p170 and p170-RBD, respectively.

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