Fig. 1.

Fig. 1. Structure simulation and expression of p170 and p170-RBD. (A) Schematic of the vaccine components. (B) Structure simulation of p170-RBD. The brown portion represents the p170 protein structure, and the blue portion represents the RBD structure. The separation of p170 and RBD proved that the addition of the linker allowed for the structural integrity of the two proteins. (C) The expression of p170 and p170-RBD in bacterial lysates. Lane 1, control group; Lane 2, p170-RBD, approximately 44 kDa; Lane 3, p170, approximately 19 kDa. (D) Western blot of p170 and p170-RBD from bacterial lysates with an anti-His monoclonal antibody. Lane 1, control group; Lane 2, p170-RBD; Lane 3, p170. Both p170 and p170-RBD had a strong reactivity with the anti-His monoclonal antibody.

© J. Microbiol. Biotechnol.