eISSN 1738-8872
pISSN 1017-7825
pISSN 1017-7825
Table. 1.
Table. 1.
Primers/probe and PCR conditions used in the present study.
| Target gene | Sequence (5'-3') | Size (bp) | PCR cycling conditions |
|---|---|---|---|
| F: AATGAACTACGAAGTGGGCG R: TCAAACGATAAAACGGTTCCTC P: FAM-ATGGTGGCGAAATGCAGAGACAGGC-BHQ1 |
112 | 50°C, 2 m→95°C, 10 m→95°C, 15 s→60°C, 1 m: 40 cycles | |
| F: GGCTTCGGTATCTGGTGGTGTA R: GGTCATTAATATTGGCCCTGAATA P:Cy5-CCACTGTCCCGTTCGTTGATGGACA-BHQ2 |
98 | 50°C, 2 m→95°C, 10 m→95°C, 15 s→60°C, 1 m: 40 cycles | |
| F: TCCATGAGAAAAGCGACTAAAAT R: AGCCGACTAATCTGTTCCTGTTC |
572 | 95°C, 3 m→95°C, 30 s→57°C, 30 s→72°C, 1 m: 30 cycles→72°C, 2 m |
J. Microbiol. Biotechnol. 2022;32:1307~1314 https://doi.org/10.4014/jmb.2207.07031
© J. Microbiol. Biotechnol.