Journal of Microbiology and Biotechnology

eISSN 1738-8872
pISSN 1017-7825

Table. 1.

Table. 1. Table 1

Bacterial strains and plasmids used in this study.

Strain/plasmid	Relevant characteristics	Source/reference
Plasmid
pESAC13	Vector used for library construction, aacIII(IV), oriT, attP-int	Bio S&T
pNPPB1s	pESAC13 with NPP B1 BGC excluding nppO, nppM, and three regulatory genes (nppRIV, nppRV, nppRVI)	This work
pUN8	pUC19 with 8 kb of the right-hand portion of NPP BGC (nppO-nppRVI)	This work
pSEN8	pSE34 with oriT and 8 kb of the right-hand portion of NPP BGC (nppO-nppRVI) containing KanR	This work
pNPPREG	pSBAC with 32 kb of the right-hand portion of BGC (part of nppC-nppRVI)	[11]
pNPPREG2	pNPPREG, which replaced Apr^R with Hyg^R	This work
pNPPREG3	pNPPREG, which replaced Apr^R with Kan^R	This work
E. coli
DH10B	E. coli host for cloning and constructs derived from various BAC vectors	Bio S&T
DH5α	E. coli host for cloning and constructs derived from various vectors	TRANS
EPI300	E. coli host for cloning and constructs derived from various vectors
ET12567/pUB307	E. coli host for transferring various plasmids into Streptomyces via conjugation
Pseudonocardia autotrophica
KCTC9441	Original NPP A1-producing strain
GG5036SP	NPP B1-producing strain (mutation in nppC)	[10]
NPP001	GG5036SP with pNPPB1s	This work
NPP002	NPP001 with pNPPREG2	This work

J. Microbiol. Biotechnol. 2022;32:911~917 https://doi.org/10.4014/jmb.2205.05036

© J. Microbiol. Biotechnol.