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Fig. 3.

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Fig. 3. Evaluation of lytic activity of CecA::ST01 against A. baumannii in vitro and in vivo. (A). The antibacterial activity of CecA::ST01 was determined using S. typhimurium ATCC 14028 and A. baumannii ATCC 17978. Each bacterial suspension in 20 mM Tris-HCl pH 7.5 was treated with 0, 0.125, 0.25, 0.5, and 1 μM of CecA::ST01. Significance is indicated as **P<0.006; *P<0.0106. Data are presented as mean ± SD (n = 3) (B). Survival rates of A. baumannii ATCC 17978 infected larvae of G. mellonella treated with no (mock) or 5 μM of CecA::ST01. The larvae were infected by 2 × 106 CFU of A. baumannii ATCC 17978. For endolysin treatment, 5 μM of CecA::ST01 was mixed with bacterial suspension immediately before injection. The survival of larvae was monitored by the time the larvae were kept at 30°C for 72 h (n = 10 per group). The experiment was repeated three times. Significance is shown as ****p < 0.0001. (C) The lytic activity of CecA::ST01 was determined using the clinical isolate of A. baumannii, CCARM 12026 by CFU reduction assay. Significance is indicated as *p < 0.0201. Data are presented as mean ± SD (n = 3) (D). The larvae of G. mellonella was infected by 2 × 106 CFU of A. baumannii CCARM 12026. For endolysin treatment, 5 μM of CecA::ST01 was mixed with bacterial suspension immediately before injection. As a control, the same amount of 1×PBS was used (mock). The survival of larvae was monitored by the time the larvae were kept at 37°C for 72 h (n = 10 per group). The experiment was repeated three times. Significance is shown as ***p < 0.0009.
J. Microbiol. Biotechnol. 2022;32:816~823 https://doi.org/10.4014/jmb.2205.05009
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